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pLenti 4.1 Ex miR200b-200a-429
(Plasmid #35533)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35533 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti 4.1Ex
  • Backbone manufacturer
    Scott Hammond
  • Backbone size w/o insert (bp) 8749
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miR200b-200a-429 genomic cluster
  • Alt name
    MIRN200B
  • Alt name
    MIRN200A
  • Alt name
    MIRN429
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2484
  • GenBank ID
    406984 406983
  • Entrez Gene
    MIR200B (a.k.a. MIRN200B, mir-200b)
  • Entrez Gene
    MIR429 (a.k.a. MIRN429, hsa-mir-429, mir-429)
  • Entrez Gene
    MIR200A (a.k.a. MIRN200A, mir-200a)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Avr11 (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti 4.1 Ex miR200b-200a-429 was a gift from Greg Goodall (Addgene plasmid # 35533 ; http://n2t.net/addgene:35533 ; RRID:Addgene_35533)
  • For your References section:

    The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Gregory PA, Bert AG, Paterson EL, Barry SC, Tsykin A, Farshid G, Vadas MA, Khew-Goodall Y, Goodall GJ. Nat Cell Biol. 2008 May;10(5):593-601. Epub 2008 Mar 30. 10.1038/ncb1722 PubMed 18376396