pLenti 4.1 Ex miR200b-200a-429
(Plasmid #35533)

• Purpose: 3rd generation lentiviral vector
• Depositing Lab: Greg Goodall
• Publication: Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. Epub 2008 Mar 30.

Backbone

• Vector backbone: pLenti 4.1Ex
• Backbone manufacturer: Scott Hammond
• Backbone size w/o insert (bp): 8749
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: miR200b-200a-429 genomic cluster
• Alt name: MIRN200B
• Alt name: MIRN200A
• Alt name: MIRN429
• Species: H. sapiens (human)
• Insert Size (bp): 2484
• GenBank ID: 406984 406983
• Entrez Gene: MIR200B (a.k.a. MIRN200B, mir-200b)
• Entrez Gene: MIR429 (a.k.a. MIRN429, hsa-mir-429, mir-429)
• Entrez Gene: MIR200A (a.k.a. MIRN200A, mir-200a)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Avr11 (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry