Tagged-IRES
(Plasmid #35570)

• Depositing Lab: Marian Waterman
• Publication: Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1.

Backbone

• Vector backbone: pRSTF
• Backbone manufacturer: Jang et al. 2004
• Backbone size w/o insert (bp): 4660
• Modifications to backbone: Renilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lymphoid Enhancer Factor 1 5'UTR
• Alt name: LEF1 IRES
• Species: H. sapiens (human)
• Insert Size (bp): 1183
• Entrez Gene: LEF1 (a.k.a. LEF-1, TCF10, TCF1ALPHA, TCF7L3)
• Promoter: SV40
• Tags / Fusion Proteins:
  • Firefly Luciferase (C terminal on insert)
  • MS2 Stem Loop (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Afe1 (destroyed during cloning)
• 3′ cloning site: Afe1 (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: LucNrev

Resource Information

• Supplemental Documents:
  • 35570 sequence
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry