pUAST_NSlmb-vhhGFP4
(Plasmid #35575)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    NSlmb-vhhGFP4
  • Alt name
    slmb
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    951
  • GenBank ID
    AY118898
  • Entrez Gene
    slmb (a.k.a. Dmel_CG3412, BcDNA:GM02031, CG3412, Dmel\CG3412, Slimb, Slimb/Beta-TRCP1, Slimb/beta-TRCP, Slmb, crd, ica, l(3)00295, shv, slimb)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer caactactgaaatctgcc
  • 3′ sequencing primer ctctgtaggtagtttgtcc
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST_NSlmb-vhhGFP4 was a gift from Markus Affolter (Addgene plasmid # 35575)
  • For your References section:

    Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Caussinus E, Kanca O, Affolter M. Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21. doi: 10.1038/nsmb.2180. 10.1038/nsmb.2180 PubMed 22157958