Addgene - pUASTattB_NSnoFbox-vhhGFP4 Plasmid Data

Plasmid 35578: pUASTattB_NSnoFbox-vhhGFP4

Gene/insert name:
NSnoFbox-vhhGFP4
Alt name:
slmb
Insert size:
795
Species:
D. melanogaster (fly)
GenBank ID:
AY118898
Entrez Gene:
slmb (Dmel_CG3412, BcDNA:GM02031, CG3412, Dmel\CG3412, Slimb, Slimb/Beta-TRCP1, Slimb/beta-TRCP, Slmb, crd, ica, l(3)00295, shv, slimb)
Mutation:
F box domain of slmb deleted.
Vector backbone:
pUASattB
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
8500
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
caactactgaaatctgcc List of Sequencing Primers
3' sequencing primer:
ctctgtaggtagtttgtcc
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Markus Affolter
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Caussinus et al (Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21. doi: 10.1038/nsmb.2180. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35578" in your Materials and Methods section.