Addgene - pPTD-DRBD Plasmid Data

Plasmid 35622: pPTD-DRBD

Gene/insert name:
PKR DRBD-1
Alt name:
double-stranded RNA-binding domain
Insert size:
210
Fusion protein or tag:
TAT
Terminal:
N terminal on backbone
Fusion protein or tag:
TAT
Terminal:
N terminal on backbone
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Fusion protein or tag:
TAT
Terminal:
N terminal on backbone
Fusion protein or tag:
6xHIS
Terminal:
C terminal on backbone
Vector backbone:
pTAT2.2
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #35621
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5500
Modifications to Backbone:
T7 tag through NotI site of MCS are replaced with TAT-HA-TAT-DRBD.
Promoter:
T7
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 fwd List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Supplemental document:
pPTD-DRBD insert sequence (image/tiff)
Principal Investigator:
Steven Dowdy
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein. Eguchi et al (Nat Biotechnol. 2009 Jun;27(6):567-71. Epub 2009 May 17. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35622" in your Materials and Methods section.