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Plasmid 35622: pPTD-DRBD
  • PKR DRBD-1

  • double-stranded RNA-binding domain

  • 210

  • TAT

  • N terminal on backbone

  • TAT

  • N terminal on backbone

  • HA

  • N terminal on backbone

  • TAT

  • N terminal on backbone

  • 6xHIS

  • C terminal on backbone

  • pTAT2.2
    (Search Vector Database)

  • Addgene plasmid #35621

  • Bacterial Expression

  • 5500

  • T7 tag through NotI site of MCS are replaced with TAT-HA-TAT-DRBD.

  • T7

  • None

  • Unknown

  • NotI

  • No

  • T7 fwd List of Sequencing Primers

  • Kanamycin

  • DH5alpha

  • 37

  • Unknown

  • View sequences (2)
  • pPTD-DRBD insert sequence (image/tiff)

  • Steven Dowdy

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein. Eguchi et al (Nat Biotechnol. 2009 Jun;27(6):567-71. Epub 2009 May 17. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35622" in your Materials and Methods section.