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pPTD-DRBD
(Plasmid #35622)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pTAT2.2
  • Backbone manufacturer
    Addgene plasmid #35621
  • Backbone size w/o insert (bp) 5500
  • Modifications to backbone
    T7 tag through NotI site of MCS are replaced with TAT-HA-TAT-DRBD.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    PKR DRBD-1
  • Alt name
    double-stranded RNA-binding domain
  • Insert Size (bp)
    210
  • Promoter T7
  • Tags / Fusion Proteins
    • TAT (N terminal on backbone)
    • TAT (N terminal on backbone)
    • HA (N terminal on backbone)
    • TAT (N terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 fwd
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPTD-DRBD was a gift from Steven Dowdy (Addgene plasmid # 35622)
  • For your References section:

    Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein. Eguchi A, Meade BR, Chang YC, Fredrickson CT, Willert K, Puri N, Dowdy SF. Nat Biotechnol. 2009 Jun;27(6):567-71. Epub 2009 May 17. 10.1038/nbt.1541 PubMed 19448630