pSV40 7xmutmiR-122T
(Plasmid
#35663)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepgl3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5256
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name7 mutant miR-122 target sites
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SpeciesSynthetic
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Insert Size (bp)153
- Promoter SV-40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR V (not destroyed)
- 3′ cloning site Apa I (not destroyed)
- 5′ sequencing primer ttggagcacggaaagacgatg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSV40 7xmutmiR-122T was a gift from Phillip Zamore (Addgene plasmid # 35663 ; http://n2t.net/addgene:35663 ; RRID:Addgene_35663)