|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35686||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerWeinberg (www.addgene.org/1764/)
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)483
GenBank ID407020 NR_029502.1
Entrez GeneMIR28 (a.k.a. MIRN28, hsa-mir-28, miR-28)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pBABE-5
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE-miR-28 was a gift from George Calin (Addgene plasmid # 35686)
For your References section:Strand-Specific miR-28-5p and miR-28-3p Have Distinct Effects in Colorectal Cancer Cells. Almeida MI, Nicoloso MS, Zeng L, Ivan C, Spizzo R, Gafa R, Xiao L, Zhang X, Vannini I, Fanini F, Fabbri M, Lanza G, Reis RM, Zweidler-McKay PA, Calin GA. Gastroenterology. 2012 Jan 10. 10.1053/j.gastro.2011.12.047 PubMed 22240480
Generated by Addgene from plasmid data.