pBABE-miR-28
(Plasmid #35686)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Weinberg (www.addgene.org/1764/)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    hsa-miR-28
  • Alt name
    MIR28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    483
  • GenBank ID
    407020 NR_029502.1
  • Entrez Gene
    MIR28 (a.k.a. MIRN28, hsa-mir-28, miR-28)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-miR-28 was a gift from George Calin (Addgene plasmid # 35686)
  • For your References section:

    Strand-Specific miR-28-5p and miR-28-3p Have Distinct Effects in Colorectal Cancer Cells. Almeida MI, Nicoloso MS, Zeng L, Ivan C, Spizzo R, Gafa R, Xiao L, Zhang X, Vannini I, Fanini F, Fabbri M, Lanza G, Reis RM, Zweidler-McKay PA, Calin GA. Gastroenterology. 2012 Jan 10. 10.1053/j.gastro.2011.12.047 PubMed 22240480