Addgene - pJC53.2/Smed001246 Plasmid Data

Plasmid 35693: pJC53.2/Smed001246

Gene/insert name:
Protein arginine methyltransferase 1 homolog
Alt name:
Smed001246
Insert size:
800
Species:
S. mediterranea
GenBank ID:
JQ035531
Vector backbone:
pJC53.2
(Search Vector Database)
Backbone manufacturer:
Jim Collins, Newmark lab
Promoter:
Sp6 for sense, T7 for sense and antisense
Cloning method:
TOPO Cloning
5' sequencing primer:
Sp6 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin and Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Phillip Newmark
Terms and Licenses:
MTA

Comments:

There are several mismatches between Addgene's quality control sequence and the available NCBI sequence. Depositing lab believes these mismatches will not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells. Rouhana et al (Development. 2012 Mar;139(6):1083-94. Epub 2012 Feb 8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35693" in your Materials and Methods section.