Addgene - pEGFP-N1-human lyn(C468A)

Plasmid 35959: pEGFP-N1-human lyn(C468A)–GFP

Gene/insert name:
LYN
Insert size:
1555
Species:
H. sapiens (human)
GenBank ID:
Entrez Gene:
LYN (JTK8, p53Lyn, p56Lyn)
Fusion protein or tag:
EGFP
Terminal:
C terminal on insert
Mutation:
c468a
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Modifications to Backbone:
NA
Promoter:
cmv
Cloning site 5':
Nhe I
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Anna Huttenlocher
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lyn is a redox sensor that mediates leukocyte wound attraction in vivo. Yoo et al (Nature. 2011 Nov 20;480(7375):109-12. doi: 10.1038/nature10632. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35959" in your Materials and Methods section.