Addgene - pCR-blunt-TOPO-FNDC5 Plasmid Data

Plasmid 35970: pCR-blunt-TOPO-FNDC5

Gene/insert name:
fibronectin type III domain containing 5
Alt name:
Fndc5, PeP, Pxp, C87088, AI836596, 1500001L03Rik
Insert size:
630
Species:
M. musculus (mouse)
GenBank ID:
NM_027402
Entrez Gene:
Fndc5 (RP23-284L6.4, 1500001L03Rik, AI836596, C87088, PeP, Pxp)
Vector backbone:
pCR-Blunt II-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
cloning vector
Backbone size w/o insert (bp):
3519
Cloning method:
TOPO Cloning
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (1)
Principal Investigator:
Bruce Spiegelman
Terms and Licenses:
MTA

Comments:

The Fndc5 gene has a high GC content and therefore may be difficult to re-clone by PCR, restriction digest should be used in re-cloning attempts.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Bostrom et al (Nature. 2012 Jan 11;481(7382):463-8. doi: 10.1038/nature10777. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35970" in your Materials and Methods section.