pcDNA3.1-TAT-2-130-FLAG
(Plasmid #35984)

• Depositing Lab: Susana Valente
• Publication: Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-108.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: transactivating protein
• Alt name: tat; p14
• Insert Size (bp): 432
• Mutation: tat from HIV2 130 amino acids
• GenBank ID: 155871
• Entrez Gene: tat (a.k.a. HIV1gp5)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry