Addgene - Park2_L (TAL 3012) Plasmid Data

Plasmid 36004: Park2_L (TAL 3012)

Gene/insert name:
TAL array targeting Park2
Alt name:
Park2
Species:
D. rerio (zebrafish)
Entrez Gene:
park2 (si:ch211-123f21.1, zgc:112390)
Fusion protein or tag:
3X Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
WT FOKI
Terminal:
C terminal on backbone
Vector backbone:
JDS 74
(Search Vector Database)
Vector type:
Mammalian Expression ; T7
Cloning site 5':
NA
Site destroyed during cloning:
Unknown
Cloning site 3':
NA
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-f List of Sequencing Primers
3' sequencing primer:
BGH rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Addgene's sequencing results identified a single nucleotide mismatch at bp# 3873 when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, this mismatch does not affect the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36004" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

36005: Park2_R (TAL 3013)

40210: pExodus CMV.Trex2