Addgene - Reporter Plasmid Data

Plasmid 36008: Reporter

Gene/insert name:
LAP2β-TM
Fusion protein or tag:
11kD FRB
Terminal:
N terminal on insert
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Mutation:
FRB is residues 2025-2114 of FRAP, LAP2β-TM is residues 401-452 of LAP2β
Vector backbone:
pEGFP-N3
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression ; Reporter
Backbone size w/o insert (bp):
4700
Promoter:
CMV
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV fwd List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Larry Gerace
Terms and Licenses:
MTA

Comments:

To be used in conjunction with protein trap construct (Addgene plasmid #36009).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore. Ohba et al (J Cell Biol. 2004 Dec 20;167(6):1051-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36008" in your Materials and Methods section.