Addgene - pcDNA3.1 MCS-BirA(R118G)-HA Plasmid Data

Plasmid 36047: pcDNA3.1 MCS-BirA(R118G)-HA

Gene/insert name:
BirA(R118G)-HA
Insert size:
993
Species:
E. coli
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
R118G
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5333
Modifications to Backbone:
Replaces MCS
Cloning method:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Kyle Roux
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Roux et al (J Cell Biol. 2012 Mar 12. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36047" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

35700: pcDNA3.1 mycBioID

40259: pcDNA3-Clover

40260: pcDNA3-mRuby2

31310: pLOVE-miR302/367

27794: mVenus C1