Gene/insert name: unc5b
Alt name: unc5b
Insert size: 1150
Species:
D. rerio (zebrafish)
Entrez Gene:
unc5b
(unc5h2)
Fusion protein or tag: ratCD4d3+4
Terminal: C terminal on backbone
Fusion protein or tag: pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP)
Terminal: C terminal on backbone
Fusion protein or tag: beta-lactamase
Terminal: C terminal on backbone
Mutation: L2V
Vector backbone:
pTT3Search Vector Database )
Backbone manufacturer:
Yves Durocher, NRC
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6000
Promoter:
CMV promoter
Promoter: CMV
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
AscI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Foward
List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
Sequence:
View sequences (3)
Principal Investigator:
Gavin Wright
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
36149" in your Materials and Methods section.
This plasmid contains an intentional L2V mutation in the unc5b sequence to create a consensus Kozak sequence.