pEGFP-Sec16A
(Plasmid #36155)

• Purpose: Expresses Sec16A as a GFP fusion
• Depositing Lab: David Stephens
• Publication: Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 2006 Sep 27.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Difficult to grow and prep without apparent loss of some of insert.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sec16A
• Alt name: KIAA0310
• Species: H. sapiens (human)
• Entrez Gene: SEC16A (a.k.a. KIAA0310, SEC16L, p250)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GGGGAGATCTATGCAGCCACCGCCCCAGACGG
• 3′ sequencing primer: GGGGGAATTCTCAGTTCAGCACCAGGTGCTTCC

Resource Information

• Supplemental Documents:
  • EGFP-Sec16A cloning info
• A portion of this plasmid was derived from a plasmid made by: Original insert from Kasuza as detailed in the attachment. Cloned by Peter Watson, now at University of Cardiff when he was a postdoc in the lab.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a D1555N mutation in Sec16A compared to the NCBI reference sequence NP_055681.1, this mutation is not known to affect plasmid function.