pEYFP-Sec16A
(Plasmid
#36156)
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PurposeExpresses Sec16A as a YFP fusion
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEYFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec16A
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Alt nameKIAA0310
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SpeciesH. sapiens (human)
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Entrez GeneSEC16A (a.k.a. KIAA0310, SEC16L, p250)
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Tag
/ Fusion Protein
- EYFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned by Peter Watson, now at University of Cardiff when he was a postdoc in the lab.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEYFP-Sec16A was a gift from David Stephens (Addgene plasmid # 36156 ; http://n2t.net/addgene:36156 ; RRID:Addgene_36156) -
For your References section:
Sec16 defines endoplasmic reticulum exit sites and is required for secretory cargo export in mammalian cells. Watson P, Townley AK, Koka P, Palmer KJ, Stephens DJ. Traffic. 2006 Dec;7(12):1678-87. Epub 2006 Sep 27. 10.1111/j.1600-0854.2006.00493.x PubMed 17005010