Addgene - pCY 3090-02 Plasmid Data

Plasmid 36231: pCY 3090-02

Gene/insert name:
yEpolylinker
Insert size:
34
Fusion protein or tag:
yEpolylinker-mCherry-hphMX4
Terminal:
C terminal on backbone
Vector backbone:
pBS35
(Search Vector Database)
Backbone manufacturer:
Yeast Resource Center, University of Washington
Vector type:
Yeast cassette for PCR and homologous recombination
Backbone size w/o insert (bp):
4872
Cloning site 5':
XmaI
Site destroyed during cloning:
Unknown
Cloning site 3':
PacI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CCTTATGTATCATACACATACG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5alpha used for DNA recovery of plasmid (i.e. DNA preps).
High or low copy:
Unknown
Selectable markers:
Hygromycin
Person or lab that originally
cloned the gene/insert:
yEpolylinker synthesized; however, the parental vector contains mCherry for which a MTA is needed (R. Tsien lab) -please see http://depts.washington.edu/yeastrc/pages/pBS35.html
Sequence: View sequences (3)
Supplemental document:
Theoretical sequence (application/pdf)
Principal Investigator:
Anne Robinson
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Young et al (Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr 4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36231" in your Materials and Methods section.