Addgene - pCY 3090-07 Plasmid Data

Plasmid 36232: pCY 3090-07

Gene/insert name:
Streptoalloteichus hindustanus bleomycin (Sh ble)
Insert size:
970
Species:
Streptoalloteichus hindustanus
Fusion protein or tag:
yEpolylinker-mCherry-Zeocin
Terminal:
C terminal on backbone
Vector backbone:
pCY 3090-02
(Search Vector Database)
Vector type:
Yeast cassette for PCR and homologous recombination
Cloning site 5':
BglII
Site destroyed during cloning:
Unknown
Cloning site 3':
SacI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CACCATCGTGGAACAGTACG List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5alpha used for DNA recovery of plasmid (i.e. DNA preps).
High or low copy:
Unknown
Selectable markers:
Zeocin
Person or lab that originally
cloned the gene/insert:
pPICZ (invitrogen); however, the parental vector contains mCherry for which a MTA is needed (R. Tsien lab) -please see http://depts.washington.edu/yeastrc/pages/pBS35.html
Sequence: View sequences (3)
Supplemental document:
Theoretical sequence (application/pdf)
Principal Investigator:
Anne Robinson
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Young et al (Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr 4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36232" in your Materials and Methods section.

Price: $65.00

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