Addgene - pRibo EcoHind Plasmid Data

Plasmid 36252: pRibo EcoHind

Gene/insert name:
None
Vector backbone:
pMV261
(Search Vector Database)
Backbone manufacturer:
Stover et al., 1991 Nature 351:456
Vector type:
Bacterial Expression ; Mycobacteria replicating vector
Modifications to Backbone:
The plasmid is derived from pMV261 (http://www.lablife.org/p?a=vdb_view&id=g2.7281169b992e40c865698d3455e3633c24b04b31). It contains a modified promoter.
Promoter:
Modified Hsp60
3' sequencing primer:
rrnB-T1-term-Rev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Carolyn Bertozzi
Principal Investigator:
Jessica Seeliger
Terms and Licenses:
MTA

Comments:

This plasmid is related to pRibo BsaHind, but is not reported in PMID 2227955333: Unlike pRibo BsaHind, the original MCS is preserved, except that the BstBI site is lost.

The plasmid is high copy in E. coli, but low copy in mycobacteria.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al (PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36252" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

17972: pSE100

17973: pTE-4S-0X

32362: pMN437