Addgene - HA-mDyn3 pcDNA3 Plasmid Data

Plasmid 36265: HA-mDyn3 pcDNA3

Gene/insert name:
Dynamin 3
Alt name:
Dyn3
Alt name:
mDyn3
Species:
M. musculus (mouse)
Entrez Gene:
Dnm3 (9630020E24Rik, AW061159, B230343F03Rik, C530045C17, mKIAA0820)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Bacterial Expression
Promoter:
CMV
Cloning site 5':
unknown
Site destroyed during cloning:
Unknown
Cloning site 3':
unknown
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Pietro De Camilli
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Ferguson et al (Science. 2007 Apr 27;316(5824):570-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36265" in your Materials and Methods section.