Addgene - pcDNA-ZifCY1 Plasmid Data

Plasmid 36315: pcDNA-ZifCY1

Gene/insert name:
ZifCY1 genetically encoded Zn(II) sensor
Alt name:
Cys2His2 sensor
Insert size:
1515
Species:
Synthetic
Vector backbone:
pcDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Promoter:
CMV
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 Forward List of Sequencing Primers
3' sequencing primer:
BGH Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Amy Palmer
Terms and Licenses:
MTA

Comments:

First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Genetically encoded sensors to elucidate spatial distribution of cellular zinc. Dittmer et al (J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36315" in your Materials and Methods section.