Addgene - pSMP-DNMT1_3 Plasmid Data

Plasmid 36384: pSMP-DNMT1_3

Gene/insert name:
DNMT1
shRNA sequence:
GAGTCTGGCTTTGAGAGTT
Species:
H. sapiens (human)
Entrez Gene:
DNMT1 (AIM, CXXC9, DNMT, HSN1E, MCMT)
Vector backbone:
pSMP
(Search Vector Database)
Backbone manufacturer:
Steve Elledge
Vector type:
Mammalian Expression, Retroviral, RNAi
Backbone size w/o insert (bp):
6699
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pSMP-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
George Daley
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chromatin-modifying enzymes as modulators of reprogramming. Onder et al (Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36384" in your Materials and Methods section.