pHAGE hSPC-dsRed Express-W
(Plasmid #36449)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Richard Mulligan
  • Backbone size w/o insert (bp) 4874
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert 1

  • Gene/Insert name
    human SP-C promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3813
  • Promoter human SP-C promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer ACGGACACATATAAGACCCTGGTCACA
  • 3′ sequencing primer GGAGCAACATAGTTAAGAATACCAGTCAATCTTTC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    dsRed Express
  • Insert Size (bp)
    677
  • Promoter N/A

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (unknown if destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer CCATTATCGTTTCAGACCCACCTCC
  • 3′ sequencing primer TCGCCGTCGAACTTCACCTCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human 3.7 kb SP-C promoter obtained from Whitsett Lab (Whitsett 3.7SPC/SV40 plasmid).
  • Terms and Licenses

Depositor Comments

Cloned h3.7kb SP-C promoter (from Whitsett 3.7SPC/SV40 plasmid)
into pHAGE-CMV-GFP-W as follows:

SV40t removed from SPC plasmid by BamH1-BamH1 cutting, blunting and self-ligtion of blunt ends. Entire hSPC promoter lifted out by cutting Nde1-Not1 and ligated into pHAGE CMV-GFP-W backbone pre-cut Nde1-Not1 to remove most of CMV promoter. Cut this backbone Spe1-Nde1 to remove residual CMV promoter fragment, blunted and ligated
NB: final vector still has Spe1 site at start of promoter.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE hSPC-dsRed Express-W was a gift from Darrell Kotton (Addgene plasmid # 36449)
  • For your References section:

    Efficient derivation of purified lung and thyroid progenitors from embryonic stem cells. Longmire TA, Ikonomou L, Hawkins F, Christodoulou C, Cao Y, Jean JC, Kwok LW, Mou H, Rajagopal J, Shen SS, Dowton AA, Serra M, Weiss DJ, Green MD, Snoeck HW, Ramirez MI, Kotton DN. Cell Stem Cell. 2012 Apr 6;10(4):398-411. 10.1016/j.stem.2012.01.019 PubMed 22482505