Addgene - TAL2275 Plasmid Data

Plasmid 36712: TAL2275

Gene/insert name:
MDM2-TALEN_Right
Alt name:
MDM2
Insert size:
1538
Species:
H. sapiens (human)
Entrez Gene:
Mdm2 (1700007J15Rik, AA415488, Mdm-2)
Fusion protein or tag:
3X Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
WT FOKI
Terminal:
C terminal on backbone
Vector backbone:
JDS70
(Search Vector Database)
Backbone manufacturer:
Joung lab (Addgene Plasmid # 32285)
Vector type:
Mammalian, T7 for in vitro mRNA synthesis
Backbone size w/o insert (bp):
6447
Promoter:
CMV
Cloning site 5':
BsmBI
Site destroyed during cloning:
Yes
Cloning site 3':
BsmBI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Target binding site: TCGCTGCCGGGCCAGTA

It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FLASH assembly of TALENs for high-throughput genome editing. Reyon et al (Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36712" in your Materials and Methods section.