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pBT259_(pRosa26-G-tTA2)
(Plasmid #36878)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36878 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pROSA26-PA (Addgene plasmid Plasmid 21271)
  • Backbone size w/o insert (bp) 8774
  • Total vector size (bp) 15069
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    If insufficient amounts of plasmid obtained from DH5alpha, we recommend Stbl2 (Invitrogen) cells grown at 30C.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Alt name
    mut4EGFP
  • Mutation
    deleted nucleotides after nucleotide 274
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tTA2
  • Alt name
    tetracycline transactivator
  • Mutation
    removed start codon
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    beta-globin intron
  • Mutation
    insertion of FRT, lox5171, lox2272, FRT, loxP-Neo-loxP
  • Promoter CAG (chicken beta actin promoter and CMV enhancer)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Neo
  • Alt name
    neomycin phosphotransferase
  • Alt name
    neomycin resistance gene
  • Promoter pPGK (phosphoglycerate kinase promoter)

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    diphteria toxin A
  • Promoter SV40

Cloning Information for Gene/Insert 5

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    W. Hillen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT259_(pRosa26-G-tTA2) was a gift from Liqun Luo (Addgene plasmid # 36878 ; http://n2t.net/addgene:36878 ; RRID:Addgene_36878)
  • For your References section:

    Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386