Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

beta-arrestin2 mYFP
(Plasmid #36917)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36917 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 zeo
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-arrestin2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1900
  • Entrez Gene
    Arrb2 (a.k.a. BARRES)
  • Promoter CMV
  • Tag / Fusion Protein
    • mYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    beta-arrestin2 mYFP was a gift from Robert Lefkowitz (Addgene plasmid # 36917 ; http://n2t.net/addgene:36917 ; RRID:Addgene_36917)
  • For your References section:

    G-protein-coupled receptor kinase specificity for beta-arrestin recruitment to the beta2-adrenergic receptor revealed by fluorescence resonance energy transfer. Violin JD, Ren XR, Lefkowitz RJ. J Biol Chem. 2006 Jul 21;281(29):20577-88. Epub 2006 May 10. 10.1074/jbc.M513605200 PubMed 16687412