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GST-beta-arrestin1
(Plasmid #36918)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36918 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-arrestin1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Arrb1 (a.k.a. BARRES)
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX5'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-beta-arrestin1 was a gift from Robert Lefkowitz (Addgene plasmid # 36918 ; http://n2t.net/addgene:36918 ; RRID:Addgene_36918)
  • For your References section:

    The active conformation of beta-arrestin1: direct evidence for the phosphate sensor in the N-domain and conformational differences in the active states of beta-arrestins1 and -2. Nobles KN, Guan Z, Xiao K, Oas TG, Lefkowitz RJ. J Biol Chem. 2007 Jul 20;282(29):21370-81. Epub 2007 May 18. 10.1074/jbc.M611483200 PubMed 17513300