Addgene - pWPXL-c-Myc Plasmid Data

Plasmid 36980: pWPXL-c-Myc

Gene/insert name:
c-Myc
Species:
M. musculus (mouse)
Entrez Gene:
Myc (AU016757, Myc2, Niard, Nird, bHLHe39)
Vector backbone:
pWPXL
(Search Vector Database)
Backbone manufacturer:
Trono lab (Addgene plasmid 12257)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
10510
Promoter:
EF1a
Cloning site 5':
MluI
Site destroyed during cloning:
Yes
Cloning site 3':
SpeI
Site destroyed during cloning:
Yes
5' sequencing primer:
EF1a-fwd List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

The mouse c-myc cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the original vector with mouse c-myc.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Slug and Sox9 cooperatively determine the mammary stem cell state. Guo et al (Cell. 2012 Mar 2;148(5):1015-28. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36980" in your Materials and Methods section.