Addgene - pWPXL-Klf4 Plasmid Data

Plasmid 36981: pWPXL-Klf4

Gene/insert name:
Klf4
Species:
M. musculus (mouse)
Entrez Gene:
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Vector backbone:
pWPXL
(Search Vector Database)
Backbone manufacturer:
Trono lab (Addgene plasmid 12257)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
10510
Promoter:
EF1a
Cloning site 5':
MluI
Site destroyed during cloning:
Yes
Cloning site 3':
SpeI
Site destroyed during cloning:
Yes
5' sequencing primer:
EF1a-fwd List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

The mouse Klf4 cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the vector backbone with mouse Klf4.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Slug and Sox9 cooperatively determine the mammary stem cell state. Guo et al (Cell. 2012 Mar 2;148(5):1015-28. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36981" in your Materials and Methods section.