|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36981||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerTrono lab (Addgene plasmid 12257)
- Backbone size w/o insert (bp) 10510
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
SpeciesM. musculus (mouse)
Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
- Promoter EF1a
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (destroyed during cloning)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer EF1a-fwd
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
The mouse Klf4 cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the vector backbone with mouse Klf4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pWPXL-Klf4 was a gift from Bob Weinberg (Addgene plasmid # 36981 ; http://n2t.net/addgene:36981 ; RRID:Addgene_36981)
For your References section:Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965