Addgene - Desmoglein2_tail-GST Plasmid Data

Plasmid 36990: Desmoglein2_tail-GST

Gene/insert name:
Dsg2
Alt name:
Desmoglein2
Species:
H. sapiens (human)
Entrez Gene:
DSG2 (ARVC10, ARVD10, CDHF5, CMD1BB, HDGC)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
Intracellular domain of DSG2 (nucleotides 1911–3516)
Vector backbone:
PGEX4T-1
(Search Vector Database)
Backbone manufacturer:
GE Healthcare
Vector type:
Bacterial Expression
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Kathleen Green
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A role for caveolin-1 in desmoglein binding and desmosome dynamics. Brennan et al (Oncogene. 2012 Mar 29;31(13):1636-48. doi: 10.1038/onc.2011.346. Epub 2011 Aug 15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36990" in your Materials and Methods section.

Price: $65.00

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