Addgene - Desmoglein2-mCherry Plasmid Data

Plasmid 36991: Desmoglein2-mCherry

Gene/insert name:
Dsg2
Alt name:
Desmoglein2
Species:
H. sapiens (human)
Entrez Gene:
DSG2 (ARVC10, ARVD10, CDHF5, CMD1BB, HDGC)
Fusion protein or tag:
mCherry
Terminal:
C terminal on backbone
Vector backbone:
pmCherry
(Search Vector Database)
Backbone manufacturer:
K. Green lab
Vector type:
Mammalian Expression
Modifications to Backbone:
EGFP cDNA was replaced with mCherry in the pEGFP N1 vector (Takara Bio Inc.) using AgeI and BsrGI.
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
SacII
Site destroyed during cloning:
No
5' sequencing primer:
mCherry-R List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Kathleen Green
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes. Nekrasova et al (J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36991" in your Materials and Methods section.