Addgene - Desmocollin2_tail-GST Plasmid Data

Plasmid 36992: Desmocollin2_tail-GST

Gene/insert name:
Dsc2
Alt name:
Desmocollin2
Species:
H. sapiens (human)
GenBank ID:
NM_024422.3
Entrez Gene:
DSC2 (ARVD11, CDHF2, DG2, DGII/III, DSC3)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
Intracellular domain of Dsc2 (nt 2,603–3,158)
Vector backbone:
PGEX4T-1
(Search Vector Database)
Backbone manufacturer:
GE Healthcare
Vector type:
Bacterial Expression
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Kathleen Green
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes. Nekrasova et al (J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36992" in your Materials and Methods section.

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