|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37007||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro (Addgene plasmid 8453)
Backbone manufacturerWeinberg lab
- Backbone size w/o insert (bp) 7032
- Total vector size (bp) 7053
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Alt nameshRNA against mouse Sox10
SpeciesM. musculus (mouse)
Entrez GeneSox10 (a.k.a. Dom, Sox21, gt)
- Promoter hU6
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1-sh-mSox10-2 was a gift from Bob Weinberg (Addgene plasmid # 37007)
For your References section:Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965
Generated by Addgene from plasmid data.