Addgene - pLKO.1-sh-mSox10-2 Plasmid Data

Plasmid 37007: pLKO.1-sh-mSox10-2

Gene/insert name:
sh-mSox10-2
Alt name:
Sox10
Alt name:
shRNA against mouse Sox10
shRNA sequence:
GGAGGTTGCTGAACGAAAGTG
Species:
M. musculus (mouse)
Entrez Gene:
Sox10 (Dom, Sox21)
Vector backbone:
pLKO.1 puro (Addgene plasmid 8453)
(Search Vector Database)
Backbone manufacturer:
Weinberg lab
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
7032
Promoter:
hU6
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Slug and Sox9 cooperatively determine the mammary stem cell state. Guo et al (Cell. 2012 Mar 2;148(5):1015-28. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37007" in your Materials and Methods section.