pClneoFLAG mouse RASSF2
(Plasmid
#37017)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCl-neo (modified)
-
Backbone manufacturerPromega
-
Modifications to backbonepCI-neo vector was modified to include FLAG tag N-terminal to MCS.
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRASSF2
-
SpeciesM. musculus (mouse)
-
MutationD72G and S127F
-
GenBank IDNP_780654.1
-
Entrez GeneRassf2 (a.k.a. RP23-401J24.8, 3830431H01Rik, 9030412M04Rik, AI852669, AW495050)
- Promoter CMV
-
Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pClneoFLAG mouse RASSF2 was a gift from Yutaka Hata (Addgene plasmid # 37017 ; http://n2t.net/addgene:37017 ; RRID:Addgene_37017) -
For your References section:
Hippo pathway-dependent and -independent roles of RASSF6. Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K, Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y. Sci Signal. 2009 Sep 29;2(90):ra59. 10.1126/scisignal.2000300 PubMed 19797269