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pGCC4
(Plasmid #37058)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37058 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHSS6
  • Backbone manufacturer
    Addgene plasmid #37060
  • Modifications to backbone
    pGCC4 is a clone of Neisseria gonorrhoeae (Gc) chromosomal DNA containing the Cg8 transposon insertion, which does not affect recombination phenotypes (Mehr and Seifert, 1997) inserted into pHSS6 at the ClaI site.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to create strains to complement mutations using the NICS system (Mehr and Seifert, 1998; Mehr, 2000). Insert a functional copy of the gene ectopically at an unlinked chromosomal locus between the lctP and aspC genes under control of lac regulatory sequences, and linked to an Erythromycin-resistance cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGCC4 was a gift from Hank Seifert (Addgene plasmid # 37058 ; http://n2t.net/addgene:37058 ; RRID:Addgene_37058)