Addgene - pGCC5 Plasmid Data

Plasmid 37059: pGCC5

Gene/insert name:
None
Vector backbone:
pHSS6
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #37060
Vector type:
Modifications to Backbone:
pGCC5 is a clone of Neisseria gonorrhoeae (Gc) chromosomal DNA containing the Cg8 transposon insertion, which does not affect recombination phenotypes (Mehr and Seifert, 1997) inserted into pHSS6 at the ClaI site.
5' sequencing primer:
CAT-R List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Hank Seifert
Terms and Licenses:
MTA

Comments:

Used to create strains to complement mutations using the NICS system (Mehr and Seifert, 1998; Mehr, 2000). Insert a functional copy of the gene ectopically at an unlinked chromosomal locus between the lctP and aspC genes under control of its endogenous promoter and linked to a Cam resistance cassette.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37059" in your Materials and Methods section.