Addgene - pLSSmOrange-C1 Plasmid Data

Plasmid 37131: pLSSmOrange-C1

Gene/insert name:
LSSmOrange
Insert size:
710
Mutation:
A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
Vector backbone:
pC1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Promoter:
CMV
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Vladislav Verkhusha
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging. Shcherbakova et al (J Am Chem Soc. 2012 Apr 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37131" in your Materials and Methods section.