Gene/insert name: LSSmOrange
Insert size: 710
Fusion protein or tag: human alpha-tubulin
Terminal: C terminal on insert
Mutation: A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
Vector backbone:
pC1
(Search Vector Database )
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5327
Modifications to Backbone:
human alpha-tubulin insert
Promoter: CMV
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F
List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence:
View sequences (3)
Map:
View map
Principal Investigator:
Vladislav Verkhusha
Terms and Licenses:
MTA
Clontech Limited Use Label License
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
37137" in your Materials and Methods section.