pCP5b
(Plasmid #37185)

• Purpose: (Empty Backbone)
• Depositing Lab: Adam Bogdanove
• Publication: Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218

Backbone

• Vector backbone: pCP5
• Backbone manufacturer: Voytas lab (Addgene Plasmid #35397)
• Backbone size (bp): 13853
• Modifications to backbone: Plasmid pCP5 yeast 2micron origin of replication and Kanamycin resistance gene replaced with pBR322 origin of replication and Ampicilin resistance cassette from pBluescript SK (to increase replication yield)
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • Cloning Targets into pCP5b

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCP5b is a high copy derivative of pCP5 for higher DNA yield from bacteria.
Plasmid used to assay TALEN function in yeast. Clone target sequences into BglII and SpeI sites as described on article.
Grow yeast transformants in SD-Ura-Trp prior to assay to prevent spontaneous recombination between the lacZ fragments