Addgene - UbG75C Plasmid Data

Plasmid 37194: UbG75C

Gene/insert name:
Ubiquitin G75C mutant
Insert size:
228
Species:
H. sapiens (human)
Fusion protein or tag:
No tag
Mutation:
Glycine 75 changed to Cystine
Vector backbone:
PET3a
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4640
Promoter:
T7
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7term
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Start culture has to be freshly grow 4 to 6 hrs until O.D. around 0.6. Store it in 4oC overnight. Then it will be used to grow culture for expression.
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Cynthia Wolberger
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener et al (Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37194" in your Materials and Methods section.