UbG76C
(Plasmid
#37195)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 37195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePET3a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4640
- Total vector size (bp) 4868
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsStart culture has to be freshly grow 4 to 6 hrs until O.D. around 0.6. Store it in 4oC overnight. Then it will be used to grow culture for expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUbiquitin G76C mutant
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SpeciesH. sapiens (human)
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Insert Size (bp)228
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MutationGlycine 76 changed to Cystine
- Promoter T7
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Tag
/ Fusion Protein
- No tag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UbG76C was a gift from Cynthia Wolberger (Addgene plasmid # 37195) -
For your References section:
The mechanism of OTUB1-mediated inhibition of ubiquitination. Wiener R, Zhang X, Wang T, Wolberger C. Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911. 10.1038/nature10911 PubMed 22367539