pCMV-RosaR4 KKR mutations
(Plasmid #37199)

• Depositing Lab: Charles Gersbach
• Publication: Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14.

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: David J. Segal (UC Davis)
• Total vector size (bp): 5918
• Vector type: Mammalian Expression ; ZFN expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FokI (fused to RosaR4 ZFN)
• Species: Flavobacterium okeanokoites
• Mutation: I538K, E490K, and H537R
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • SV40 NLS (N terminal on backbone)
  • ROSA26 eZFNs R4 (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: pBABE 3

Resource Information

• Supplemental Documents:
  • ZFN sequences
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ZFN details can be found on the attached pdf and in the original manuscript.