pCMV-RosaR4 KKR mutations
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37199||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid J. Segal (UC Davis)
- Total vector size (bp) 5918
Vector typeMammalian Expression ; ZFN expression
Growth in Bacteria
Gene/Insert nameFokI (fused to RosaR4 ZFN)
MutationI538K, E490K, and H537R
- Promoter CMV
/ Fusion Proteins
- HA (N terminal on backbone)
- SV40 NLS (N terminal on backbone)
- ROSA26 eZFNs R4 (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer Sp6
- 3′ sequencing primer pBABE 3 (Common Sequencing Primers)
ZFN details can be found on the attached pdf and in the original manuscript.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-RosaR4 KKR mutations was a gift from Charles Gersbach (Addgene plasmid # 37199)
For your References section:Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA. Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14. 10.1093/nar/gkr1214 PubMed 22169954