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pCMV-RosaR4 KKR mutations
(Plasmid #37199)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37199 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    David J. Segal (UC Davis)
  • Total vector size (bp) 5918
  • Vector type
    Mammalian Expression ; ZFN expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FokI (fused to RosaR4 ZFN)
  • Species
    Flavobacterium okeanokoites
  • Mutation
    I538K, E490K, and H537R
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • SV40 NLS (N terminal on backbone)
    • ROSA26 eZFNs R4 (N terminal on insert)

Cloning Information

Resource Information

Depositor Comments

ZFN details can be found on the attached pdf and in the original manuscript.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-RosaR4 KKR mutations was a gift from Charles Gersbach (Addgene plasmid # 37199)
  • For your References section:

    Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases. Perez-Pinera P, Ousterout DG, Brown MT, Gersbach CA. Nucleic Acids Res. 2012 Apr 1;40(8):3741-52. Epub 2011 Dec 14. 10.1093/nar/gkr1214 PubMed 22169954