Addgene - pTYB2-PAB1 Plasmid Data

Plasmid 37234: pTYB2-PAB1

Gene/insert name:
PAB1
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
PAB1 (YER165W)
Fusion protein or tag:
intein
Terminal:
C terminal on insert
Vector backbone:
pTYB2
(Search Vector Database)
Backbone manufacturer:
NEB
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7474
Promoter:
T7
Cloning site 5':
NdeI
Site destroyed during cloning:
Unknown
Cloning site 3':
SmaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Jon Lorsch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1*PABP mRNPs in vivo. Park et al (EMBO J. 2011 Jan 19;30(2):302-16. Epub 2010 Dec 7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37234" in your Materials and Methods section.

Price: $65.00

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