Renilla luciferase-Pol III
(Plasmid #37380)

• Depositing Lab: Norbert Perrimon
• Publication: Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub 2005 Nov 20.

Backbone

• Vector backbone: pRL-null
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3320
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PolIII-Renilla control reporter
• Alt name: RNA polymerase III control promoter
• Alt name: RNA PolIII 128 subunit (RpIII128)
• Alt name: Renilla luciferase
• Species: D. melanogaster (fly)
• GenBank ID: AE003823
• Entrez Gene: RpIII128 (a.k.a. Dmel_CG8344, CG8344, DmRP128, Dmel\CG8344, RP128)
• Promoter: RNA PolIII 128 subunit (RpIII128)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Perrimon Lab plasmid #414

The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.