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Renilla luciferase-Pol III
(Plasmid #37380)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37380 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRL-null
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3320
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PolIII-Renilla control reporter
  • Alt name
    RNA polymerase III control promoter
  • Alt name
    RNA PolIII 128 subunit (RpIII128)
  • Alt name
    Renilla luciferase
  • Species
    D. melanogaster (fly)
  • GenBank ID
    AE003823
  • Entrez Gene
    RpIII128 (a.k.a. Dmel_CG8344, CG8344, DmRP128, Dmel\CG8344, RP128)
  • Promoter RNA PolIII 128 subunit (RpIII128)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site SpeI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Perrimon Lab plasmid #414

The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Renilla luciferase-Pol III was a gift from Norbert Perrimon (Addgene plasmid # 37380 ; http://n2t.net/addgene:37380 ; RRID:Addgene_37380)
  • For your References section:

    A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. Nat Genet. 2005 Dec;37(12):1323-32. Epub 2005 Nov 20. 10.1038/ng1682 PubMed 16311596