Renilla luciferase-Pol III
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37380||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3320
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePolIII-Renilla control reporter
Alt nameRNA polymerase III control promoter
Alt nameRNA PolIII 128 subunit (RpIII128)
Alt nameRenilla luciferase
SpeciesD. melanogaster (fly)
Entrez GeneRpIII128 (a.k.a. Dmel_CG8344, CG8344, DmRP128, Dmel\CG8344, RP128)
- Promoter RNA PolIII 128 subunit (RpIII128)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Perrimon Lab plasmid #414
The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Renilla luciferase-Pol III was a gift from Norbert Perrimon (Addgene plasmid # 37380 ; http://n2t.net/addgene:37380 ; RRID:Addgene_37380)
For your References section:A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. Nat Genet. 2005 Dec;37(12):1323-32. Epub 2005 Nov 20. 10.1038/ng1682 PubMed 16311596