pEV9.03
(Plasmid #37495)

• Depositing Lab: Geraldine Seydoux
• Publication: Voronina et al Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24.

Backbone

• Vector backbone: pL4440gw
• Backbone manufacturer: Caldwell(?)
• Backbone size w/o insert (bp): 2790
• Modifications to backbone: Exchanged ccdB gene in original backbone for insert
• Vector type: Worm Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: npp-16
• Species: C. elegans (nematode)
• Insert Size (bp): 511
• Mutation: nts 162-673 of cds
• Entrez Gene: npp-16 (a.k.a. Y56A3A.17)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: L4440
• 3′ sequencing primer: pBluescript-KS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry