Addgene - pRS306:Met3p-mCherry-Tub1 Plasmid Data

Plasmid 37554: pRS306:Met3p-mCherry-Tub1

Gene/insert name:
Met3p-mCherry-Tub1
Insert size:
2700
Species:
S. cerevisiae (budding yeast)
Vector backbone:
pRS306
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4381
Cloning site 5':
SalI
Site destroyed during cloning:
Yes
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (4)
Principal Investigator:
Wei-Lih Lee
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Quantitative analysis of Pac1/LIS1-mediated dynein targeting: Implications for regulation of dynein activity in budding yeast. Markus et al (Cytoskeleton (Hoboken). 2011 Mar;68(3):157-74. doi: 10.1002/cm.20502. Epub 2011 Feb 3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37554" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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