Addgene - pAcGP67A-hBAI3-HormR-GAIN Plasmid Data

Plasmid 37848: pAcGP67A-hBAI3-HormR-GAIN

Gene/insert name:
BAI3
Insert size:
1110
Species:
H. sapiens (human)
Entrez Gene:
BAI3 (KIAA0550, MGC133100)
Fusion protein or tag:
8xHIS
Terminal:
C terminal on insert
Mutation:
HormR and GAIN domains only (aa498–868
Vector backbone:
pAcGP67A
(Search Vector Database)
Backbone manufacturer:
BD Biosciences
Vector type:
Baculovirus Transfer Vectors
Backbone size w/o insert (bp):
9761
Promoter:
Polyhedrin
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
polyhedrin-fwd List of Sequencing Primers
3' sequencing primer:
polyhedrin-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Axel Brunger
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. Arac et al (EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37848" in your Materials and Methods section.

Price: $65.00

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