pPK700
(Plasmid #38146)

• Depositing Lab: Patricia Kuwabara
• Publication: Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epub 2011 Jan 4.

Backbone

• Vector backbone: pBluescript KSII(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 14610
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: let-60p::ptc-3::gfp
• Alt name: let-60 promoter
• Alt name: ptc-3
• Species: C. elegans (nematode)
• Entrez Gene: ptc-3 (a.k.a. Y110A2AL.8)
• Promoter: let-60
• Tag / Fusion Protein:
  • EGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13pUC-rev
• 3′ sequencing primer: M13pUC-fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pPK700 plasmid was derived by ligating the let-60 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145)