Spa-1 in pCCL-c-MNDU3c-(X2)-pgk-EGFP
(Plasmid #38154)

• Depositing Lab: Nora Heisterkamp
• Publication: Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Backbone

• Vector backbone: pCCL-c-mNDU3c-(X2)-Pgk-EGFP
• Backbone manufacturer: Don Kohn
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 11200
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Spa-1
• Species: H. sapiens (human)
• Insert Size (bp): 3300
• GenBank ID:
• Entrez Gene: SIPA1 (a.k.a. SPA1)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: MSCV_rev
• 3′ sequencing primer: EGFP-N

Gene/Insert 2

• Gene/Insert name: EGFP
• Promoter: PGK

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: mPGK-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: insert bought from Origene Spa-1 in pCMV-XL6
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Construct made by Sun Yu Ji. Human Spa-1 was subcloned as a 3.3 kb NotI x SspI fragment into pSK digested with NotI x SmaI; it was isolated from this plasmid by EcoRI digestion. The 3.3 kb EcoRI fragment was ligated into pCCL-c-MNDU3-(X2)-PGK-EGFP digested with EcoRI. The orientation of the insert with respect to the MNDU3 promoter was checked by digestion with Kpn I. The construct makes untagged human SpaI from the MNDU3 promoter and EGFP from the PGK promoter.